Academic Affiliation
Assistant Professor, Department of Chemistry and Biochemistry in the College of Liberal Arts and Sciences
Credentials
PhD, 1998, University of Buenos Aires
Bio
Dr. Marcia Levitus’ research is focused on the development and application of state-of-the-art techniques of single molecule detection to study complex biological systems. She uses an interdisciplinary approach that interweaves concepts from physics, chemistry and biology.
In contrast with the more conventional fluorescence techniques, where billions of molecules are sampled simultaneously, single molecule techniques allow the observation of subpopulations and rare events that would otherwise be hidden in the measured average. More importantly, the observation of an individual molecule allows for the study of dynamic aspects of conformational changes without the need to synchronize the entire sample. A related technique, fluorescence correlation spectroscopy, is based on the analysis of the fluctuations in the fluorescent signal of a small number of molecules. Correlation analysis of the fluorescence fluctuations yields kinetic information about the dynamic processes that cause the changes in the fluorescent signal.
Dr. Levitus uses these concepts to investigate the dynamics, structure and kinetics of nucleoprotein assemblies. Specific projects in this line of research include the study of the dynamic aspects of DNA-protein interactions in nucleosomes. She is interested in characterizing the spontaneous DNA unwrapping and re-wrapping kinetics and in the study of the effect of ATP-dependent remodeling enzymes.