Anb

Biodesign Directory Profile

Contact Information

(480)727-0445

Roy Curtiss, PhD

Director

The Biodesign Institute, Infectious Diseases and Vaccinology

Professor

College of Liberal Arts and Sciences, School of Life Sciences

Bio

Roy Curtiss, III, PhD, is the Director of the Centers for Infectious Diseases and Vaccinology and Microbial Genetic Engineering in the Biodesign Institute and Professor of Life Sciences in the School of Life Sciences. Curtiss is a member of the National Academy of Sciences and Fellow of the American Academy of Microbiology, the American Association for the Advancement of Science, the St. Louis Academy of Sciences and the Arizona Arts, Science and Technology Academy.

His most recent emphasis has been the design and construction of cyanobacterial strains to both maximize production of biofuels and biofuel precursors and to possess genetically controlled means to facilitate product recovery in an energy efficient manner. More specifically this includes 1) optimizing the expression of genes encoding enzymes needed for free fatty acid (FFA) production, 2) over expressing these genes by encoding them on multi- copy plasmids stabilized by balanced-lethal technologies, 3) eliminating genes encoding enzymes for competing pathways and reduction of genome size by eliminating non-essential genes and operons, 4) engineering strains and culture conditions to inhibit, if not eliminate, growth of contaminating heterotrophs that might consume produced FFAs, 5) developing means to capture FFAs concomitant with production and secretion, 6) developing a process for genetically controlled induction of autoaggregation to concentrate biomass without need for filtration and/or centrifugation, 7) developing a method for liberating membrane lipids at high temperatures and high density in the dark, and 8) developing means for biocontainment such that any escaping genetically modified cyanobacteria could not survive in the environment. Another major endeavor is directed at developing live vaccines to reduce morbidity and mortality caused by infectious disease agents of fish, poultry, swine, cattle and humans. His research group designs, constructs and evaluates recombinant genetically modified Salmonella vaccine strains as immunizing vectors to express and deliver protective antigens/epitopes representing virulence determinants from other pathogens and/or to deliver novel DNA vaccines encoding protective antigens/epitopes specified by genetic information from various pathogens for expression by the immunized individual. These recombinant attenuated Salmonella vaccines (RASVs) are administered intranasally or orally to home to the nasal-, bronchial-, and/or gut-associated lymphoid tissues and thus induce mucosal, humoral, and cellular immunities and confer protective immunity to bacterial, viral and parasite infectious disease agents infecting agriculturally important animals and humans. Current endeavors are directed at developing vaccines to prevent infections by: Streptococcus pneumoniae, Mycobacterium tuberculosis, Clostridium perfringens, Yersinia pestis, enteric pathogens (some of which cause systemic disease) such as Salmonella, Escherichia, Shigella, Camylobacter jejuni, Edwardsiella ictaluri and Yersinia, Listeria monocytogenes, influenza viruses (human and avian), papilloma virus, Giardia, Eimeria species and Taenia solium.  This research has led to an increasing interest in the ecological distribution of pathogens and their transmission and interaction with animal hosts to exert evolutionary pressures. Since much of host resistance to infection is dependent on innate and acquired immunity, many of these research efforts, especially in optimizing vaccines for maximal induction of immune protection, focus on immunological understanding of all aspects of host defense.

Selected Publications

2013

  • Brenneman, K. E., C. Willingham, W. Kong, R. Curtiss III, and K. L. Roland. Low-pH rescue of acid-sensitive Salmonella enterica Serovar Typhi strains by a rhamnose-regulated arginine decarboxylase system. J. Bacteriol. (2013) Refereed Articles.
  • Dziva, F., H. Hauser, T. Connor, P. M. van Diemen, G. Prescott, G. C. Langridge, S. Eckert, R. R. Chaudhuri, C. Ewers, M. Mellata, S. Mukhopadhyay, R. Curtiss III, G. Dougan, L. H. Wieler, N. R. Thomson, D. J. Pickard, and M. P. Stevens. Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms. Infect. Immun. (2013) Refereed Articles.
  • Editors unknown, Curtiss, R. III. Mucosal Immunology. Antigen delivery system II: Development of live recombinant attenuated bacterial vectors (2013) Book Chapters.
  • Fisher, M. L., R. Allen, Y. Luo, and R. Curtiss II. Export of extracellular polysaccharides modulates adherence of the cyanobacterium Synechocystis. PLoS One (2013) Refereed Articles.
  • Frey, S. E., K. R. Lottenbach, H. Hill, T. P. Blevins, Y. Yu, Y. Zhang, K. E. Brenneman, S. M. Kelly-Aehle, C. McDonald, A. Jansen, and R. Curtiss III. A Phase I, dose-escalation trial in adults of three recombinant attenuated Salmonella Typhi vaccine vectors producing Streptococcus pneumoniae surface protein antigen PspA. Vaccine (2013) Refereed Articles.
  • Guan, L., J. Santander, M. Mellata, Y. Zhang, and R. Curtiss III. Identification of an iron acquisition machinery in Flavobacterium columnare. Dis. Aquat. Organ. (2013) Refereed Articles.
  • Kupz, A., T. A. Scott, G. T. Belz, D. M. Andrews, M. Greyer, A. M. Lew, A. G. Brooks, M. J. Smyth, R. Curtiss III, S. Bedoui, and R. Strugnell. Contribution of Thy1+ NK cells to protective IFN-?-production during Salmonella Typhimurium infections. Proc. Natl. Acad. USA (2013) Refereed Articles.
  • Lottenbach, K. R., S. M. Kelly-Aehle, K. E. Brenneman, R. Curtiss III, and S. E. Frey. Rapid, sensitive recovery of recombinant attenuated Salmonella enterica serovar Typhi vaccine strains from human blood. Clin. Vaccine Immunol. (2013) Refereed Articles.
  • Mitra, A., A. Loh, A. Gonzales, P. Laniewski, C. Willingham, R. Curtiss III and K. L. Roland. Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens. Vaccine (2013) Refereed Articles.
  • Mitra, A., A. Loh, A. Gonzales, P. Laniewski, C. Willingham, R. Curtiss III and K. L. Roland.. Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens.. Vaccine (2013) Refereed Articles.
  • Pei, Y., V. R. Parreira, K. L. Roland, R. Curtiss III, and J. F. Prescott. Assessment of attenuated Salmonella vaccine strains in controlling experimental Salmonella Typhimurium infection in chickens. Can. Vet. J. Res. (2013) Refereed Articles.
  • Santander, J., T. Martin, A. Loh, C. Pohlenz, D. M. Gatlin 3rd, and R. Curtiss III. Mechanisms of intrinsic resistance to antimicrobial peptides of Edwardsiella ictaluri and its influence on fish gut inflammation and virulence.. Microbiology (2013) Refereed Articles.
  • Shi, H., S. Wang, and R. Curtiss III. Evaluation of regulated delayed attenuation strategies for Salmonella enterica serovar Typhi vaccine vectors in neonatal and infant mice. Clin. Vaccine Immunol. (2013) Refereed Articles.
  • Sun, W., D. A. Six, C. M. Reynolds, H. S. Chung, C. R. H. Raetz, and R. Curtiss III. Pathogenicity of Yersinia pestis synthesis of 1-dephosphorylated lipid A. Infect. Immun. (2013) Refereed Articles.
  • Wang, S., H, Shi, Y. Li, Z. Shi, X. Zhang, C. H. Baek, T. Mothershead, and R. Curtiss III. A colanic acid operon deletion mutation enhances induction of early antibody responses by live attenuated Salmonella vaccines. Infect. Immun. (2013) Refereed Articles.
  • Wang, S., Q. Kong, and R. Curtiss III. New technologies in developing recombinant attenuated Salmonella vaccine vectors. Microbial Pathog (2013) Refereed Articles.
  • Wang, S., Q. Kong, and R. Curtiss III. New technologies in developing recombinant attenuated Salmonella vaccine vectors. Microbial. Pathog. (2013) Refereed Articles.

2012

  • Brenneman, K. E., C. McDonald, S. M. Kelly, K. L. Roland, and R. Curtiss III. Use of RapidChek? SELECT Salmonella to detect shedding of live attenuated Salmonella enterica serovar Typhi vaccine strains. J. Microbiol. Methods (2012) Refereed Articles.
  • Ju?rez-Rodr?guez, M. D., J. Yang, R. Kader, P. Alamuri, R. Curtiss III, and J. E. Clark-Curtiss. Live attenuated Salmonella vaccines displaying regulated delayed lysis and delayed antigen synthesis to confer protection against Mycobacterium tuberculosis. Infect. Immun. (2012) Refereed Articles.
  • Ju?rez-Rodr?guez, M. D., L. T. Arteaga-Cort?s, R. Kader, R. Curtiss III, and J. E. Clark-Curtiss. Live attenuated Salmonella Vaccines against Mycobacterium tuberculosis with antigen delivery via the type III secretion system. Infect. Immun. (2012) Refereed Articles.
  • Kong, Q., D. Six, Q. Liu, L. Gu, S. Wang, P. Alamuri, C. R. H. Raetz, and R. Curtiss III. Phosphate groups of lipid A are essential for Salmonella enterica serovar Typhimurium virulence and affect innate and adaptive immunity. Infect. Immun. (2012) Refereed Articles.
  • Kong, W., M. Brovold, B. A. Koneneman, J. Clark-Curtiss, and R. Curtiss III. Turning self-destructing Salmonella into a universal DNA vaccine delivery platform. *Recommended by Faculty of 1000 Biology. Proc. Natl. Acad. USA. (2012) Refereed Articles.
  • Kupz, A., G. Guarda, T. Gebhardt, L. E. Sander, K. R. Short, D. A. Diavatopoulos, O. L. C. Wijburg, H. Cao, J. C. Waithman, W. Chen, D. Fernandez-Ruiz, P. G. Whitney, W. R. Heath, R. Curtiss III, J. Tschopp, R. A. Strugnell, and S. Bedoui. NLRC4 inflammasomes in dendritic cells regulate noncognate effector function by memory CD8+ T cells. Nat. Immunol. (2012) Refereed Articles.
  • Le?n, M., J. Santander, R. Curtiss III, and J. Robeson. Natural lysogenization and transduction in Salmonella enterica serovar Choleraesuis by bacteriophage P1. Res. Microbiol. (2012) Refereed Articles.
  • Liu, X and R. Curtiss III. Thermorecovery of cyanobacterial fatty acids at elevated temperatures. J. Biotechnol. (2012) Refereed Articles.
  • Luo, Y., Q. Kong, J. Yang, A. Mitra, G. Golden, S. Y. Wanda, K. L. Roland, R. V. Jensen, P. B. Ernst, and R. Curtiss III. Comparative genome analysis of the high pathogenicity Salmonella Typhimurium strain UK-1. PLoS One (2012) Refereed Articles.
  • Mellata, M., J. T. Maddux, T. Nam, N. Thomson, H. Hauser, M. P. Stevens, S. Mukhopadhyay, S. Sarker, A. Crabbe, C. A. Nickerson, J. Santander, and R. Curtiss III. New Insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.. PloS ONE (2012) Refereed Articles.
  • Santander, J., G. Golden, S. Y. Wanda, and R. Curtiss III. The Fur-regulated uptake system of Edwardsiella icataluri and its influence on pathogenesis and immunogenicity in the catfish host. Infect. Immun. (2012) Refereed Articles.
  • Seo, S., J. Kim, H. Yang, H. Kwon, J. Yang, R. Curtiss III, and M. Kweon. Effective protection against secondary pneumococcal pneumonia by oral vaccination with attenuated Salmonella delivering PspA antigen in mice. Vaccine (2012) Refereed Articles.
  • Sun, W. and R. Curtiss III. Amino acid substitutions in LcrV at putative sites of interaction with toll-like receptor II do not affect the virulence of Yersinia pestis.. Microb. Pathog. (2012) Refereed Articles.
  • Theoret, J. R., K. K. Cooper, B. Zekarias, K. L. Roland, B. F. Law, R. Curtiss III, and L. A. Joens. The Campylobacter jejuni Dps homologue is important for in vitro biofilm formation and cecal colonization of poultry, and may serve as a protective antigen for vaccination. Clin. Vaccine Immunol. (2012) Refereed Articles.
  • Xin, W., S. Y. Wanda, X. Zhang, J. Santander, G. Scarpellini, K. Ellis, P. Alamuri, and R. Curtiss III. The Asd+-DadB+ dual-plasmid system offers novel means to deliver multiple protective antigens by a recombinant attenuated Salmonella vaccine. Infect. Immun. (2012) Refereed Articles.

2011

  • Ashraf, S., W. Kong, S. Wang, J. Yang, and R. Curtiss III.. Protective cellular responses elicited by vaccination with influenza nucleoprotein delivered by a live recombinant attenuated Salmonella vaccine.. Vaccine (2011) Refereed Articles.
  • Baek, C., H. Y. Kang, K. L. Roland, and R. Curtiss III. 2011.. Lrp acts as both a positive and negative regulator for type 1 fimbriae production in Salmonella enterica serovar Typhimurium.. PLoS ONE (2011) Refereed Articles.
  • Jenikova, G., P. Hruz, M. K. Andersson, N. Tejman-Yarden, P. C. D. Ferreira, Y. S. Andersen, B. J. Davids, F. D. Gillin, S. G. Sv?rd, R. Curtiss III, and L. Eckmann.. a1-giardin based live heterologous vaccine protects against Giardia lamblia infection in a murine model.. Vaccine (2011) Refereed Articles.
  • Kong, Q., D. A. Six, K. L. Roland, Q. Liu, L. Gu, C. M. Reynolds, X. Wang, C. R. H. Raetz, and R. Curtiss III.. Salmonella synthesizing 1-dephosphorylated lipopolysaccharide exhibits low endotoxic activity while retaining its immunogenicity.. J. Immunol. (2011) Refereed Articles.
  • Kong, Q., J. Yang, Q. Liu, P. Alamuri, K. L. Roland, and R. Curtiss III.. Effect of deletion of genes involved in lipopolysaccharide core and O-antigen synthesis on virulence and immunogenicity of Salmonella enterica serovar Typhimurium.. Infect. Immun. (2011) Refereed Articles.
  • Liu, X., J. Sheng, and R. Curtiss III. Fatty acid production in genetically modified cyanobacteria. Proc. Natl. Acad. Sci. USA (2011) Refereed Articles.
  • Liu, X., J. Sheng, and R. Curtiss III.. Fatty acid production in genetically modified cyanobacteria. Proc. Natl. Acad. Sci. USA. (2011) Refereed Articles.
  • Liu, X., S. Fallon, J. Sheng, and R. Curtiss III. CO2 limitation inducible green recovery of fatty acids from cyanobacterial biomass. Proc. Natl. Acad. Sci. USA (2011) Refereed Articles.
  • Liu, X., S. Fallon, J. Sheng, and R. Curtiss III.. CO2 limitation inducible green recovery of fatty acids from cyanobacterial biomass.. Proc. Natl. Acad. Sci. USA. (2011) Refereed Articles.
  • Luo, Y., Q. Kong, J. Yang, G. Golden, S. Y. Wanda, R. V. Jensen, P. B. Ernst, and R. Curtiss III.. Complete genome sequence of the universal killer, Salmonella enterica serovar Typhimurium UK-1 (ATCC 68169).. J. Bacteriol. (2011) Refereed Articles.
  • Santander, J., A. Mitra, and R. Curtiss III.. Phenotype, virulence and immunogenicity of Edwardsiella ictaluri cyclic adenosine 3',5'-monophosphate receptor protein (Crp) mutants in catfish host.. Fish and Shellfish Immunology (2011) Refereed Articles.
  • Sun, W, D. Six, X. Kuang, K. L. Roland, C. R. Raetz, and R. Curtiss III.. A live attenuated strain of Yersinia pestis KIM as a vaccine against plague. Vaccine (2011) Refereed Articles.
  • Sun, W., K. L. Roland, and R. Curtiss.. Developing live vaccines against Yersinia pestis.. J. Infect. Dev. Countries. (2011) Refereed Articles.
  • Wang, S., Y. Li, H. Shi, W. Sun, K. L. Roland, and R. Curtiss III.. Comparison of a regulated delayed antigen synthesis system with in vivo-inducible promoters for antigen delivery by live attenuated Salmonella vaccines.. Infect. Immun. (2011) Refereed Articles.
  • Zhang X, S. Y. Wanda, K. Brenneman, W. Kong, X. Zhang, K. Roland, and R. Curtiss III.. Improving Salmonella vector with rec mutation to stabilize the DNA cargoes.. BMC Microbiol. (2011) Refereed Articles.

2010

  • Ameiss, K., S. Ashraf, W. Kong, A. Pekosz, W. H. Wu, D. Milich, J. N. Billaud, and Roy Curtiss III.. Delivery of woodchuck hepatitis virus-like particle presented influenza M2e by recombinant attenuated Salmonella displaying a delayed lysis phenotype.. Vaccine (2010) Refereed Articles.
  • Branger, C. G., W. Sun, A. Torres-Escobar, R. Perry, K. L. Roland, J. Fetherston, and R. Curtiss III.. Evaluation of Psn, HmuR and a modified LcrV protein delivered to mice by live attenuated Salmonella as a vaccine against bubonic and pneumonic Yersinia pestis challenge.. Vaccine (2010) Refereed Articles.
  • Chow, K. C., X. Wang, R. Curtiss III, and C. Castillo-Chavez.. Evaluating the efficacy of antimicrobial cycling programs and patient isolation on dual resistance in hospitals.. J. Biol. Dyn. (2010) Refereed Articles.
  • Curtiss, R. III, W. Xin, Y. Li, W. Kong, S. Y. Wanda, B. M. Gunn, and S. Wang.. New technologies in using recombinant attenuated Salmonella vaccine vectors.. Crit. Rev. Immunol. (2010) Refereed Articles.
  • Curtiss, R. III.. The impact of vaccines and vaccinations: challenges and opportunities for modelers.. Math. Biosci. Eng. (2010) Refereed Articles.
  • Gunn, B. M., S. Y. Wanda, D. Burshell, C. Wang, and R. Curtiss III. Construction of recombinant attenuated Salmonella enterica serovar Typhimurium vaccine vector strains for safety in newborn and infant mice.. Clin. Vaccine. Immunol. (2010) Refereed Articles.
  • Hapfelmeier, S., M. A. Lawson, E. Slack, J. K. Kirundi, M. Stoel, M. Heikenwalder, J. Cahenzli, Y. Velykoredko, M. L. Balmer, K. Endt, M. B. Geuking, R. Curtiss III, K. D. McCoy, and A. J. Macpherson.. Reversible microbial colonization of germ-free mice reveals the dynamics IgA immune responses.. Science (2010) Refereed Articles.
  • Kong, Q., Q. Liu, A. M. Jansen, and R. Curtiss III.. Regulated delayed expression of rfc enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica vaccines.. Vaccine (2010) Refereed Articles.
  • Mellata, M., K. Ameiss, H. Mo, and R. Curtiss III.. Characterization of the contribution to virulence of three large plasmids of avian pathogenic E. coli (7122 (O78:K80:H9).. Infect. Immun. (2010) Refereed Articles.
  • Muralinath, M., M. J. Kuehn, K. L. Roland, and R. Curtiss III.. Immunization with Salmonella enterica serovar Typhimurium-derived outer membrane vesicles delivering the pneumococcal protein PspA confers protection against challenge with Streptococcus pneumoniae.. Infect. Immun. (2010) Refereed Articles.
  • R. Curtiss et al.. Escherichia coli and Salmonella: Cellular and Molecular Biology. (2010) Edited Books.
  • Santander, J., and R. Curtiss III. 2010.. Salmonella enterica serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of S. Typhimurium.. . Infect. Devel. Countries (2010) Refereed Articles.
  • Santander, J., W. Xin, Z. Yang, and R. Curtiss III.. Aspartate-semialdehyde dehydrogenase (asdA) gene of Edwardsiella ictaluri and its use to develop a balanced-lethal vector-host system for construction of antibiotic-sensitive live attenuated recombinant vaccines for the catfish aquaculture industry.. PLoS One (2010) Refereed Articles.
  • Shi, H., J. Santander, K. Brenneman, S. Y. Wanda, S. Wang, P. Senechal, W. Sun, K. Roland, and R. Curtiss III.. Live recombinant Salmonella Typhi vaccines constructed to investigate the role of rpoS in eliciting immunity to a heterologous antigen.. PLoS One (2010) Refereed Articles.
  • Shi, H., S. Wang, K. L. Roland, B. M. Gunn, and R. Curtiss III.. Immunogenicity of a live recombinant Salmonella enterica serovar Typhimurium vaccine expressing pspA in neonates and infant mice born from na?ve and immunized mothers.. Clin. Vaccine Immunol. (2010) Refereed Articles.
  • Sun, W., K. Roland, X. Kuang, C. G. Branger, and R. Curtiss III.. Yersinia pestis with regulated delayed attenuation as a vaccine candidate to induce protective immunity against plague.. Infect. Immun. (2010) Refereed Articles.
  • Torres-Escobar, A., M. D. Juarez-Rodriguez, B. M. Gunn, C. G. Branger, S. A. Tinge, and R. Curtiss III.. Fine-tuning synthesis of Yersinia pestis LcrV from runaway-like replication balanced-lethal plasmid in a Salmonella enterica serovar Typhimurium vaccine induces protection against a lethal Y. pestis challenge in mice.. Infect. Immun. (2010) Refereed Articles.
  • Torres-Escobar, A., M. D. Juarez-Rodriguez, C. G. Branger, and R. Curtiss III.. Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA.. Vaccine (2010) Refereed Articles.
  • Torres-Escobar, A., M. Ju?rez-Rodriguez, and R. Curtiss III.. Biogenesis of Yersinia pestis PsaA in recombinant attenuated Salmonella Typhimurium vaccine (RASV) strain.. FEMS Microbiol. Lett. (2010) Refereed Articles.
  • Wang, S., Y. Li, G. Scarpellini, W. Kong, H. Shi, C. Baek, B. Gunn, S. Y. Wanda, K. L. Roland, X. Zhang, P. Senechal-Willis, and R. Curtiss III.. Salmonella vaccine vectors displaying delayed antigen synthesis in vivo to enhance immunogenicity.. Infect. Immun (2010) Refereed Articles.
  • Wang, S., Y. Li, H. Shi, G. Scarpellini, A. Torres-Escobar, K. L. Roland, and R. Curtiss III.. Immune responses to recombinant pneumococcal PsaA antigen delivered by a live attenuated Salmonella vaccine.. Infect. Immun. (2010) Refereed Articles.

2007

  • J Santander, S Wanda, C Nickerson, Roy Curtiss. Role of RpoS in fine-tuning the synthesis of Vi capsular polysaccharide in Salmonella enterica serotype Typhi. Infect Immun (2007) Refereed Articles.

2006

  • H Loessner, A Endmann, M Rhode, Roy Curtiss, S Weiss. Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains. FEMS Microbiol. Lett (2006) Refereed Articles.
  • V Konjufca, S Wanda, M Jenkins, Roy Curtiss. Recombinant attenuated Salmonella typhimurium vaccine encoding Eimeria acervulina antigen offers protection against Eimeria acervulina challenge. Infect Immun (2006) Refereed Articles.

2005

  • M Lamarche, C Dozois, F Daigle, M Caza, Roy Curtiss, J Dubreuil, J Harel. Inactivation of the Pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain. Infect. Immun (2005) Refereed Articles.
  • Mestecky, J, Lamm, M E, Strober, W, Bienenstock, J, McGhee, J R, Mayer, L, Curtiss, Roy (Author). Mucosal Immunology. Antigen delivery systems: Development of live recombinant attenuated bacterial antigen and DNA vaccine delivery vector vaccines (2005) Book Chapters.
  • S Abromaitis, S Faucher, M Beland, Roy Curtiss, F Daigle. The presence of the tet gene from cloning vectors impairs Salmonella survival in macrophages. FEMS Microbiol. Lett (2005) Refereed Articles.
  • S Faucher, Roy Curtiss, F Daigle. Selective capture of Salmonella enterica serovar typhi genes expressed in macrophages that are absent from the Salmonella enterica serovar Typhimurium genome. Infect. Immun (2005) Refereed Articles.
  • S Uzzau, G Marogna, G Leori, Roy Curtiss, G Schianchi, B Stocker, S Rubino. Virulence attenuation and live vaccine potential of aroA, crp cdt cya, and plasmid-cured mutants of Salmonella enterica serovar Abortusovis in mice and sheep. Infect. Immun (2005) Refereed Articles.

2004

  • D Baud, J Benyacoub, M Kok, F Ponci, M Bobst, Roy Curtiss, P De Grandi, D Nardhelli-Haefliger. Immunogenicity against human papillomavirus type 16 virus like particles is strongly enhanced by the PhoPc phenotype in Salmonella typhimurium. Infect. Immun (2004) Refereed Articles.

2003

  • H Kang, Roy Curtiss. Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization. FEMS Immunol. Med. Microbiol. Lett (2003) Refereed Articles.
  • M Mellata, M Dho-Moulin, C Dozois, Roy Curtiss, B Lehoux, J Fairbrother. Role of avian pathogenic Escherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages. Infect. Immun (2003) Refereed Articles.
  • M Mellata, M Dho-Moulin, C Dozois, Roy Curtiss, P Brown, P Arne, A Bree, C Desautels, J Fairbrother. Role of virulence factors in resistance of avian pathogenic Escherichia coli to serum and in pathogenicity. Infect. Immun (2003) Refereed Articles.

2002

  • A Honeyman, C Cote, Roy Curtiss. Construction of transcriptional and translational lacZ gene reporter plasmids for use in Streptococcus mutans. J. Microbiol. Meth (2002) Refereed Articles.
  • A Schnapp, C Eickhoff, D Sizemore, Roy Curtiss, D Hoft. Cruzipain induces both mucosal and systemic protection against Trypanosoma cruzi in mice. Infect. Immun (2002) Refereed Articles.
  • D McVey, M Chengappa, D Mosier, G Stone, R Oberst, M Sylte, N Gabbert, S Kelly-Aehle, Roy Curtiss. Immunogenicity of ?4127 phoP - Salmonella enterica serovar Typhimurium in dogs. Vaccine (2002) Refereed Articles.
  • H Kang, C Dozois, S Tinge, T Lee, Roy Curtiss. Transduction-mediated transfer of unmarked deletion and point mutations through use of counterselectable suicide vectors. J. Bacteriol (2002) Refereed Articles.
  • H Kang, J Srinivasan, Roy Curtiss. Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar typhimurium vaccine. Infect. Immun (2002) Refereed Articles.
  • Roy Curtiss. Perspective: Bacterial infectious disease control by vaccine development. J. Clin. Invest (2002) Refereed Articles.

2001

  • A Sheoran, J Timoney, S Tinge, P Sundaram, Roy Curtiss. Intranasal immunogenicity of a ?cya ?crp-pabA mutant of Salmonella enterica serovar Typhimurium for the horse. Vaccine (2001) Refereed Articles.
  • F Daigle, J Graham, Roy Curtiss. Identification of Salmonella typhi genes expressed within macrophages by selective capture of transcribed sequences (SCOTS). Mol. Microbiol (2001) Refereed Articles.
  • M Schaechter, M Riley, J Beckwith, Roy Curtiss, W Hatfield, J Ingraham, K Low, B Magasanik, F Neidhardt, W Reznikoff. Escherichia coli and Salmonella 2000: The view from here. Microbiol. Mol. Biol. Rev (2001) Refereed Articles.
  • P Brown, C Dozois, C Nickerson, A Zuppardo, J Terlonge, Roy Curtiss. MlrA a novel regulator of curli (Agf) synthesis and aggregation by Escherichia coli and Salmonella enterica serovar Typhimurium. Mol. Microbiol (2001) Refereed Articles.
  • Roy Curtiss, S Blower, K Cooper, D Russell, S Silverstein, L Young. Leprosy research in the post-genome era. Lepr. Rev (2001) Refereed Articles.
  • S Frey, W Bollen, D Sizemore, M Campbell, Roy Curtiss. Bacteremia associated with live attenuated ?8110 Salmonella enterica serovar Typhi ISP1820 in healthy adult volunteers. Clin. Immunol (2001) Refereed Articles.

2000

  • A Honeyman, Roy Curtiss. The mannitol-specific enzyme II (mtIA) gene and the mtIR gene of the PTS of Streptococcus mutans. Microbiology (2000) Refereed Articles.
  • C Dozois, M Dho-Moulin, A BrĂ©e, J Fairbrother, C Desautels, Roy Curtiss. Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli, and localization and analysis of the tsh genetic region. Infect. Immun (2000) Refereed Articles.
  • C Stathopoulos, D Hendrixson, D Thanassi, S Hultgren, J St Geme, Roy Curtiss. Secretion of virulence determinants by the general secretory pathway in Gram-negative pathogens: an evolving story. Microbes Infect (2000) Refereed Articles.
  • J Maurer, T Doggett, L Burns-Keliher, Roy Curtiss. Expression of the rfa, LPS biosynthesis promoter in Salmonella typhimurium during invasion of intestinal epithelial cells. Curr. Microbiol (2000) Refereed Articles.
  • Roy Curtiss, S Blower, K Cooper, D Russell, S Silverstein, L Young. Leprosy research in the post-genome era: Workshop. Int. J. Lepr (2000) Refereed Articles.